RNA Preparation and Purification
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (200 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.
![Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-R2051DIRECTZOLRNAMINIPREPYES.JPG-250.jpg)
Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Zyagen Labs Mouse Universal Reference Total RNA extracted from wide variety of male and female C57BL/6 Mouse tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR and many other applications. 2X0.1mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
Macherey-Nagel NucleoSpin RNA Clean-up, 250 preps
NucleoSpin RNA Clean-up (250) 250 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers
![Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502037264.JPG-250.jpg)
Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]
The extraction method inactivates viruses and other infectious agents. Total RNA (including small/microRNAs) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, DNA/RNA Shield™ samples, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRIzol/TRI Reagent, wash and elute the RNA. No chloroform, phase separation or precipitation steps are necessary. High-quality total RNA is ready for Next-Gen sequencing, RT-qPCR, transcription profiling, hybridization, etc
Agilent Technologies ABSOLUTE RNABETA MINIPREP KIT
Absolutely RNAbeta RT-PCR Miniprep Kit 50 preps Total RNA purification kit for DNA-free preps; ideal for RT-PCR Contains: Prefilter spin cups RNA binding spin cups Lysis buffer beta- mercaptoethanol DNase (lyophilized) DNase buffers Wash bu
New England Biolabs, Inc. Monarch Spin RNA Cleanup Kit (500 ug) - 100 preps
The Monarch Spin RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our columns help ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch Spin RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience
Zymo Research Corporation RNA Clean & Concentrator™-5 (200 Preps) w/ Zymo-Spin™ IC Columns (Capped)
This 5 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin™ technology that allows for selective recovery of total RNA (> 17nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).The procedure is easy: Add binding buffer and ethanol to your sample,then bind, wash and elute ultra-pure RNA. The RNA can be eluted from the Zymo-Spin™ IC Column in as little as 6 l of RNase-free water. The highly concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Norgen Biotek Corp RNase-Free DNase I Kit, 50 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Sigma Aldrich Fine Chemicals Biosciences RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
Clontech Labs 3P NucleoSpin RNA Blood Midi | 20 Preps
The NucleoSpin RNA Blood Midi kit enables efficient recovery of high-quality RNA from up to 1.3 ml of whole blood using a simple and convenient procedure that can be performed at room temperature. The kit protocol is compatible with common blood collection tubes and anticoagulants—including EDTA, citrate, and heparin—and enables processing of 6 samples in 75 minutes.
Norgen Biotek Corp RNase-Free DNase I Kit, 200 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Macherey-Nagel NucleoSpin RNA, 50 preps
NucleoSpin RNA (50) 50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase