RNA Preparation and Purification
Promega PolyATtract™ System 1000 without Magnetic Stand
The PolyATtract System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolation.
Promega Biotinylated Oligo(dT) Probe (50pmol/μL)
The PolyATtract System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolation.
G-Biosciences RNA-Conserve™ Solution
RNA-Conserve™ Solution for preserving RNA.
ATCC C2BBe1 clone of Caco-2; Colon Adenocarcinoma; Human (Homo sapiens)
For-profit Organizations: For every order of this item, you must work directly with Abcam Inc, whom hold exclusive commercial distribution rights to this material from Memorial Sloan Kettering Cancer Center to (i) negotiate a research-use license, and/or (ii) have Abcam provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from Abcam that we are authorized to ship each order. We are providing the following contact information, but this information may change without notice: Abcam Inc. Phone: +44(0) 1223 696000 Email: licensing@abcam.com. Once ATCC has received authorization from Abcam Inc, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team.
Beckman Coulter RNAClean XP, 40 mL
RNAClean XP 40 mL reagent kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions.
Zymo Research Corporation Quick-RNA™ MiniPrep Plus Kit (50 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The Quick-RNA Miniprep Plus Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (500 ug) - 100 preps
The Monarch Spin RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our columns help ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch Spin RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience
Zymo Research Corporation RNA Clean & Concentrator™-5 (200 Preps) w/ Zymo-Spin™ IC Columns (Capped)
This 5 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin™ technology that allows for selective recovery of total RNA (> 17nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).The procedure is easy: Add binding buffer and ethanol to your sample,then bind, wash and elute ultra-pure RNA. The RNA can be eluted from the Zymo-Spin™ IC Column in as little as 6 l of RNase-free water. The highly concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Zymo Research Corporation Direct-zol™ RNA MicroPrep (200 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
Sigma Aldrich Fine Chemicals Biosciences RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
Macherey-Nagel NUCLEOSPIN RNA CLEAN UP MXI KT
NucleoSpin RNA Clean up Maxi kit for RNA clean up and concentration
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (50 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (50 ug) rapidly and reliably purifies up to 50 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in downstream applications including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 10 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Macherey-Nagel NucleoSpin RNA, 50 preps
NucleoSpin RNA (50) 50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Macherey-Nagel NucleoSpin RNA Plant, 50 preps
NucleoSpin RNA Plant (50) 50 preps for the isolation of RNA from plant - NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase